Respiratory sampling for severe acute respiratory syndrome coronavirus 2: An Overview

Head Neck. 2020 Jul;42(7):1652-1656. doi: 10.1002/hed.26232. Epub 2020 May 8.

Abstract

The novel coronavirus disease 2019 is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and was declared a pandemic in March 2020. A plethora of respiratory sampling methods for SARS-CoV-2 viral detection has been used and in the current evolving situation, there is no international consensus on the recommended method of respiratory sampling for diagnosis. Otolaryngologists deal intimately with the upper respiratory tract and a clear understanding of the respiratory sampling methods is of paramount importance. This article aims to provide an overview of the various methods and their evidence till date.

Keywords: COVID-19; RT-PCR; SARS-CoV-2; coronavirus; respiratory sampling.

MeSH terms

  • Betacoronavirus / genetics
  • Betacoronavirus / isolation & purification*
  • Bronchoalveolar Lavage
  • COVID-19
  • Coronavirus Infections / diagnosis*
  • Humans
  • Intubation, Intratracheal / instrumentation
  • Nasal Cavity / virology
  • Nasopharynx / virology
  • Oropharynx / virology
  • Pandemics
  • Pneumonia, Viral / diagnosis*
  • RNA, Viral / analysis
  • SARS-CoV-2
  • Specimen Handling / instrumentation
  • Specimen Handling / methods*
  • Sputum / virology
  • Therapeutic Irrigation
  • Tongue / virology
  • Trachea / virology

Substances

  • RNA, Viral