Evaluation of six anti-SARS-CoV-2 antibody test kits and practical approaches to optimize the diagnostic performance

J Microbiol Immunol Infect. 2021 Oct;54(5):983-986. doi: 10.1016/j.jmii.2021.03.008. Epub 2021 Mar 26.

Abstract

In an investigation of six anti-SARS-CoV-2 antibody kits with different target antigen and methodology, each kit showed comparable performance. As false-positive reactions occurred independently with different kits, specificity increased to 100% when pairs of kits were used. With three-kit combination, both sensitivity (99.1%) and specificity (100%) increased.

Keywords: Antibody; COVID-19; Combination; Performance; SARS-CoV-2.

MeSH terms

  • Antibodies, Viral / immunology*
  • COVID-19 / diagnosis*
  • COVID-19 Serological Testing / methods*
  • False Positive Reactions
  • Humans
  • Reagent Kits, Diagnostic*
  • Real-Time Polymerase Chain Reaction / methods
  • SARS-CoV-2 / isolation & purification*
  • Sensitivity and Specificity

Substances

  • Antibodies, Viral
  • Reagent Kits, Diagnostic